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KMID : 1007520100190010175
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Food Science and Biotechnology
2010 Volume.19 No. 1 p.175 ~ p.184
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Selection of Psychrotrophic Leuconostoc spp. from Native Fruits, and Studies on Their Dextransucrases
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Cigdem Ileri Yamaner
Ismail Yavuz Sezen
Aziz Tanriseven
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Abstract
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Present study was aimed at selection of psychrotrophic Leuconostoc spp., having dextransucrase activity from native fruits and sauerkraut. Fifty-six colonies of lactic acid bacteria producing dextransucrase were isolated and characterized by physiological-biochemical methods. Seven isolates with high dextransucrase activities (0.68-1.63 IU/mL) were studied in detail including characterization by 16S rDNA sequence comparison. It was found that these isolates grow faster and produced dextransucrase earlier than Leuconostoc mesenteroides B512FMC-13Glc, a mutant form of the commercial strain. Molecular weights of the dextransucrases produced by 7 isolates were determined and acceptor reactions using maltose were studied. Dextranase hydrolysis products of dextrans produced by new enzymes were similar to those of commercially produced B-512 F dextran. The new dextransucrases could find industrial uses and the strains isolated (AN 39-1, Or2p-2, Mu3, and Mu10) could be used as prebiotic producing starters in food fermentations due to having high dextransucrase activity and their growth at 4oC.
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KEYWORD
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acceptor reaction, dextransucrase, fruit, Leuconostoc mesenteroides, psychrotroph
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